000			01852cam a22003494a 4500
001			vtls000298173
003			UG-KaMUL
005			20250612232218.0
010			_a2007-042261
020			_a9780763742973 (alk. paper)
020			_a076374297X (alk. paper)
039			_y 201107201238 _z959
040			_aDNLM/DLC _c DLC _d NLM _d YDXCP _d BTCTA _d BAKER _d C#P _d DLC _d U G-KaMUL
082			_a572.8/6 _2 22
092			_a572.86 DNA
245			_aDNA sequencing III : _bdealing with difficult templates / _cedited by Jan Kieleczawa.
246			_aDNA sequencing three
246			_aDNA sequencing 3
260			_aSudbury, Mass. : _bJones and Bartlett Publishers, _c c2009.
300			_axvi, 184 p., 8 p. of plates : _bill. (some col.) ; _c 24 cm.
504			_aIncludes bibliographical references and index.
505			_aControlled heat-denaturation of DNA plasmids and PCR fragments -- He at denaturation is an effective step in sequencing of many difficult DN A templates -- Solutions for sequencing difficult regions -- Improving sequence results from difficult templates with Phi 29 DNA polymerase an d nucleotide analogs: the TempliPhi sequence resolver kit -- Sequencing through various secondary structures: detailed studies of pDEST vector s and other DNA templates with hairpins -- Transcriptional sequencing a s a tool for reading difficult-to-read templates -- Bias-free cloning o f "unclonable" DNA for simplified genomic finishing -- Bioinformatics t ools to aide sequencing of difficult templates.
650			_aDNA _x analysis.
650			_aNucleotide sequence _x Methodology.
650			_a Sequence Analysis, DNA _x methods.
650			_a Base Sequence.
650			_a Templates, Genetic.
700			_aKieleczawa, Jan.
942			_2ddc _cBK
999			_c498019 _d498019