Includes bibliographical refrences.

Preface -- 1 Cell line development -- 2 Transient recombinant protei n expression in mammalian cells -- 3 Hybridoma technology for the in vi tro production of therapeutic antibodies -- 4 Bioreactors for mammalian cells -- 5 Mass Transfer and Mixing Across the Scales in Animal Cell C ulture -- 6 Hydrodynamic Damage to Animal Cells -- 7 Monitoring of cell culture -- 8 Serum and protein free media -- 9 Glycosylation in Cell C ulture -- 10 Modelling of mammalian cell cultures -- 11 Mammalian Cell Line Selection Strategies for High-Producers -- 12 Building a Cell Cult ure Process with Stable Foundations: Searching for Certainty in an Unce rtain World -- 13 Perfusion Processes -- 14 Single-use bioreactors for animal and human cells -- 15 An Overview of Cell Culture Engineering fo r the Insect Cell-Baculovirus Expression Vector System (BEVS) -- 16 Met abolic flux analysis: a powerful tool in animal cell culture -- 17 Cell immobilization for the production of viral vaccines -- 18 Cell Enginee ring for Therapeutic Protein Production -- 19 Proteomics in Cell Cultur e: From genomics to combined 'omics for cell line engineering and biopr ocess development -- 20 Metabolomics in animal cell culture -- 21 Proce ss Analytical Technology and Quality-by-Design for Animal Cell Culture -- 22 Biosafety recommendations on the handling of animal cell cultures -- 23 Biopharmaceutical products from animal cell culture -- Index.

Animal cells are the preferred "cell factories" for the production o f complex molecules and antibodies for use as prophylactics, therapeuti cs or diagnostics. Animal cells are required for the correct post-trans lational processing (including glycosylation) of biopharmaceutical prot ein products. They are used for the production of viral vectors for gen e therapy. Major targets for this therapy include cancer, HIV, arthriti s, cardiovascular and CNS diseases and cystic fibrosis. Animal cells ar e used as in vitro substrates in pharmacological and toxicological stud ies. This book is designed to serve as a comprehensive review of animal cell culture, covering the current status of both research and applica tions. For the student or R&D scientist or new researcher the protocols are central to the performance of cell culture work, yet a broad under standing is essential for translation of laboratory findings into the i ndustrial production. Within the broad scope of the book, each topic is reviewed authoritatively by experts in the field to produce state-of-t he-art collection of current research. A major reference volume on cell culture research and how it impacts on production of biopharmaceutical proteins worldwide, the book is essential reading for everyone working in cell culture and is a recommended volume for all biotechnology libr aries.

Description based on publisher-supplied MARC data.