TY  - BOOK
AU  - Sambrook, Joseph.
AU  - Russell, David W.
AU  - Sambrook, Joseph.
TI  -  The condensed protocols from Molecular cloning : a laboratory manual
SN  - 0879697725 (hardcover : alk. paper)
U1  - 572.8 22
PY  - 2006///
CY  - Cold Spring Harbor, N.Y.
PB  - Cold Spring Harbor Laboratory Press
KW  - Molecular cloning
KW  -  Laboratory manuals
KW  - Cloning, Molecular
KW  -  Laboratory Manuals
N1  - "A single-volume adaptation of the three-volume third edition of Mol ecular cloning: a laboratory manual"--P. [4] of cover; Includes bibliographical references and index; Plasmids and their usefulness in molecular cloning -- Bacteriophage and its vectors -- Working with bacteriophage M13 vectors -- Working wi th high-capacity vectors -- Gel electrophoresis of DNA and pulsed-field agarose gel electrophoresis -- Preparation and analysis of eukaryotic genomic DNA -- Extraction, purification, and analysis of mRNA from euka ryotic cells -- In vitro amplification of DNA by the polymerase chain r eaction -- Preparation of radiolabeled DNA and RNA probes -- Working wi th synthetic oligonucleotide probes -- Preparation of cDNA libraries an d gene identification -- DNA sequencing -- Mutagenesis -- Screening exp ression libraries -- Expression of cloned genes in escherichia coli -- Introducing cloned genes into cultured mammalian cells -- Analysis of g ene expression in cultured mammalian cells -- Protein interaction techn ologies
UR  -  http://www.loc.gov/catdir/toc/ecip0517/20050220 77.html
ER  -