The condensed protocols from Molecular cloning : a laboratory manual - Sambrook, Joseph; Russell, David W. (David William), 1957-; Sambrook, Joseph (Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, 2006)
Plasmids and their usefulness in molecular cloning -- Bacteriophage and its vectors -- Working with bacteriophage M13 vectors -- Working wi th high-capacity vectors -- Gel electrophoresis of DNA and pulsed-field agarose gel electrophoresis -- Preparation and analysis of eukaryotic genomic DNA -- Extraction, purification, and analysis of mRNA from euka ryotic cells -- In vitro amplification of DNA by the polymerase chain r eaction -- Preparation of radiolabeled DNA and RNA probes -- Working wi th synthetic oligonucleotide probes -- Preparation of cDNA libraries an d gene identification -- DNA sequencing -- Mutagenesis -- Screening exp ression libraries -- Expression of cloned genes in escherichia coli -- Introducing cloned genes into cultured mammalian cells -- Analysis of g ene expression in cultured mammalian cells -- Protein interaction techn ologies.
"A single-volume adaptation of the three-volume third edition of Mol ecular cloning: a laboratory manual"--P. [4] of cover.
Includes bibliographical references and index.
Keywords: Molecular cloning -- Laboratory manuals; Cloning, Molecular -- Laboratory Manuals