TY  - BOOK
AU  - Kieleczawa, Jan.
TI  - DNA sequencing III: dealing with difficult templates 
SN  - 9780763742973 (alk. paper)
U1  - 572.8/6  22
PY  - 2009///
CY  - Sudbury, Mass.
PB  - Jones and Bartlett Publishers
KW  - DNA
KW  -  analysis
KW  - Nucleotide sequence
KW  -  Methodology
KW  -  Sequence Analysis, DNA
KW  -  methods
KW  -  Base Sequence
KW  -  Templates, Genetic
N1  - Includes bibliographical references and index; Controlled heat-denaturation of DNA plasmids and PCR fragments -- He at denaturation is an effective step in sequencing of many difficult DN A templates -- Solutions for sequencing difficult regions -- Improving sequence results from difficult templates with Phi 29 DNA polymerase an d nucleotide analogs: the TempliPhi sequence resolver kit -- Sequencing through various secondary structures: detailed studies of pDEST vector s and other DNA templates with hairpins -- Transcriptional sequencing a s a tool for reading difficult-to-read templates -- Bias-free cloning o f "unclonable" DNA for simplified genomic finishing -- Bioinformatics t ools to aide sequencing of difficult templates
ER  -