DNA sequencing III : dealing with difficult templates - Kieleczawa, Jan. (Sudbury, Mass. : Jones and Bartlett Publishers, 2009)
Controlled heat-denaturation of DNA plasmids and PCR fragments -- He at denaturation is an effective step in sequencing of many difficult DN A templates -- Solutions for sequencing difficult regions -- Improving sequence results from difficult templates with Phi 29 DNA polymerase an d nucleotide analogs: the TempliPhi sequence resolver kit -- Sequencing through various secondary structures: detailed studies of pDEST vector s and other DNA templates with hairpins -- Transcriptional sequencing a s a tool for reading difficult-to-read templates -- Bias-free cloning o f "unclonable" DNA for simplified genomic finishing -- Bioinformatics t ools to aide sequencing of difficult templates.
Includes bibliographical references and index.
Keywords: DNA -- analysis; Nucleotide sequence -- Methodology; Sequence Analysis, DNA -- methods; Base Sequence; Templates, Genetic