DNA sequencing III :  dealing with difficult templates / 
 DNA sequencing three  DNA sequencing 3 
edited by Jan Kieleczawa. 
 - Sudbury, Mass. :  Jones and Bartlett Publishers,   c2009. 
 - xvi, 184 p., 8 p. of plates :  ill. (some col.) ;   24 cm. 
<br/><br/>Includes bibliographical references and index. 
<br/><br/>Controlled heat-denaturation of DNA plasmids and PCR fragments -- He at denaturation is an effective step in sequencing of many difficult DN A templates -- Solutions for sequencing difficult regions -- Improving sequence results from difficult templates with Phi 29 DNA polymerase an d nucleotide analogs: the TempliPhi sequence resolver kit -- Sequencing through various secondary structures: detailed studies of pDEST vector s and other DNA templates with hairpins -- Transcriptional sequencing a s a tool for reading difficult-to-read templates -- Bias-free cloning o f "unclonable" DNA for simplified genomic finishing -- Bioinformatics t ools to aide sequencing of difficult templates. 
<br/><br/><label>ISBN: </label>9780763742973 (alk. paper) 076374297X (alk. paper) 
<br/><br/><label>LCCN: </label>2007-042261 
<br/><br/><label>Subjects--Topical Terms: </label><br/>DNA-- analysis.<br/>Nucleotide sequence-- Methodology.<br/> Sequence Analysis, DNA-- methods.<br/> Base Sequence.<br/> Templates, Genetic.
<br/><br/><label>Dewey Class. No.: </label>572.8/6