Rev. ed. of: Recombinant gene expression protocols / edited by Rocky S. Tuan. c1997.

Includes bibliographical references and index.

Host cell compatibility in protein expression / James J. Greene -- P roduction of recombinant proteins / Laura A. Palomares, Sandino Estrada -Mondaca, and Octavio T. Ramâirez -- Folding promoting agents in recomb inant protein production / Beatrix Fahnert -- Back to basics / Paulina Balbâas and Francisco Bolivar -- Alpha-complementation enabled t7 expre ssion vectors and their use for the expression of recombinant polypepti des for protein transduction experiments / Mikhail F. Alexeyev, Viktori ya V. Pastukh, Inna N. Shokolenko, and Glenn L. Wilson -- Expression of recombinant alkaline phosphatase conjugates in Escherichia coli / Jean -Claude Boulain and Frâedâeric Ducancel -- Overexpression of chromosoma l genes in Escherichia coli / Fernando Valle and Noemâi Flores -- Chrom osomal expression of foreign and native genes from regulatable promoter s in Escherichia coli / Lu Zhou, Ke Zhang and Barry L. Wanner --

Plasmid vectors for marker-free chromosomal insertion of genetic mat erial in Escherichia coli / Sylvie Le Borgne, Francisco Bolivar, and Gu illermo Gosset -- Copy-control pBAC/oriV vectors for genomic cloning / Jadwiga Wild and Waclaw Szybalski -- Copy-control tightly regulated exp ression vectors based on pBAC/oriV / Jadwiga Wild and Waclaw Szybalski -- Cell-free protein synthesis with prokaryotic combined transcription- translation / James R. Swartz, Michael C. Jewett, and Kim A. Woodrow-Mu mford -- Genetic tools for the manipulation of moderately halophilic ba cteria of the family halomonadaceae / Carmen Vargas and Joaquâin J. Nie to -- Gene transfer and expression of recombinant proteins in moderatel y halophilic bacteria / Amalia S. Afendra, Carmen Vargas, Joaquâin J. N ieto and Constantin Drainas -- Recombinant protein production in antarc tic gram-negative bacteria / Angela Duilio, Maria Luisa Tutino, and Gen naro Marino -- Recombinant protein production in yeasts / Danilo Porro and Diethard Mattanovich -- Controlled expression of homologous genes b y genomic promoter replacement in the yeast Saccharomyces cerevisiae / Kevin J. Vestrepen and Johan M. Thevelein -- High-throughput expression in microplate format in saccharomyces cerevisiae / Caterina Holz and C hristine Lang -- High-throughput expression in microplate format in Pic hia pastoris / Mewes Bèotner and Christine Lang -- Multiple gene expres sion by chromosomal integration and CRE-lox mediated marker recycling i n saccharomyces cerevisiae / Bjèorn Johansson and Bèarbel Hahn-Hèagerda l --

Three decades of fungal transformation / Vianey Olmedo-Monfil, Carlo s Cortâes-Penagos, and Alfredo Herrera-Estrella -- Three decades of fun gal transformation / Sergio Casas-Flores, Teresa Rosales-Saavedra, and Alfredo Herrera-Estrella -- Gene transfer and expression in plants / Ar gelia Lorence and Robert Verpoorte -- Production of recombinant protein s by hairy roots cultured in plastic sleeve bioreactors / Fabricio Medi na-Bolâivar and Carole Cramer -- Engineering the chloroplast genome for hyperexpression of human therapeutic proteins and vaccine antigens / Shashi Kumar and Henry Daniell -- New selection marker for plant transf ormation / Barbara Leyman, Nelson Avonce, Matthew Ramon, Patrick Van Di jck, Johan M. Thevelein, and Gabriel Iturriaga -- Enhancer detection an d gene trapping a functional genomics in plants / Gerardo Acosta-Garc âia, Daphnâe Autran, and Jean-Philippe Vielle-Calzada -- Gene transfer and expression in mammalian cell lines and transgenic animals / Felix R ecillas-Targa -- Sustained heterologous transgene expression in mammali an and avian cell lines / Hâector Rincâon-Arano and Felix Recillas-Targ a -- Inducible gene expression in mammalian cells and mice / Wilfried W ebber and Martin Fussenegger -- Flp-mediated integration of expression cassettes into FRT-tagged chromosomal lociin mammalian cells / Dagmar W irth and Hansjèorg Hauser -- Generation of high recombinant protein pro ducing Chinese hamster ovary cells / Jean-Louis Goergen and Lucâia Mona co -- Preparation of recombinant proteins in milk / Louis-Marie Houdebi ne.