The condensed protocols from Molecular cloning : a laboratory manual /
Sambrook, Joseph.
The condensed protocols from Molecular cloning : a laboratory manual / Joseph Sambrook, David W. Russell. - Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, c2006. - v, 800 p. : ill. ; 28 cm.
"A single-volume adaptation of the three-volume third edition of Mol ecular cloning: a laboratory manual"--P. [4] of cover.
Includes bibliographical references and index.
Plasmids and their usefulness in molecular cloning -- Bacteriophage and its vectors -- Working with bacteriophage M13 vectors -- Working wi th high-capacity vectors -- Gel electrophoresis of DNA and pulsed-field agarose gel electrophoresis -- Preparation and analysis of eukaryotic genomic DNA -- Extraction, purification, and analysis of mRNA from euka ryotic cells -- In vitro amplification of DNA by the polymerase chain r eaction -- Preparation of radiolabeled DNA and RNA probes -- Working wi th synthetic oligonucleotide probes -- Preparation of cDNA libraries an d gene identification -- DNA sequencing -- Mutagenesis -- Screening exp ression libraries -- Expression of cloned genes in escherichia coli -- Introducing cloned genes into cultured mammalian cells -- Analysis of g ene expression in cultured mammalian cells -- Protein interaction techn ologies.
0879697725 (hardcover : alk. paper) 0879697717 (pbk. : alk. paper) 9780879697716
2005-022077
Molecular cloning-- Laboratory manuals.
Cloning, Molecular-- Laboratory Manuals.
572.8
The condensed protocols from Molecular cloning : a laboratory manual / Joseph Sambrook, David W. Russell. - Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, c2006. - v, 800 p. : ill. ; 28 cm.
"A single-volume adaptation of the three-volume third edition of Mol ecular cloning: a laboratory manual"--P. [4] of cover.
Includes bibliographical references and index.
Plasmids and their usefulness in molecular cloning -- Bacteriophage and its vectors -- Working with bacteriophage M13 vectors -- Working wi th high-capacity vectors -- Gel electrophoresis of DNA and pulsed-field agarose gel electrophoresis -- Preparation and analysis of eukaryotic genomic DNA -- Extraction, purification, and analysis of mRNA from euka ryotic cells -- In vitro amplification of DNA by the polymerase chain r eaction -- Preparation of radiolabeled DNA and RNA probes -- Working wi th synthetic oligonucleotide probes -- Preparation of cDNA libraries an d gene identification -- DNA sequencing -- Mutagenesis -- Screening exp ression libraries -- Expression of cloned genes in escherichia coli -- Introducing cloned genes into cultured mammalian cells -- Analysis of g ene expression in cultured mammalian cells -- Protein interaction techn ologies.
0879697725 (hardcover : alk. paper) 0879697717 (pbk. : alk. paper) 9780879697716
2005-022077
Molecular cloning-- Laboratory manuals.
Cloning, Molecular-- Laboratory Manuals.
572.8