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DNA sequencing III : dealing with difficult templates / DNA sequencing three DNA sequencing 3 edited by Jan Kieleczawa. - Sudbury, Mass. : Jones and Bartlett Publishers, c2009. - xvi, 184 p., 8 p. of plates : ill. (some col.) ; 24 cm.

Includes bibliographical references and index.

Controlled heat-denaturation of DNA plasmids and PCR fragments -- He at denaturation is an effective step in sequencing of many difficult DN A templates -- Solutions for sequencing difficult regions -- Improving sequence results from difficult templates with Phi 29 DNA polymerase an d nucleotide analogs: the TempliPhi sequence resolver kit -- Sequencing through various secondary structures: detailed studies of pDEST vector s and other DNA templates with hairpins -- Transcriptional sequencing a s a tool for reading difficult-to-read templates -- Bias-free cloning o f "unclonable" DNA for simplified genomic finishing -- Bioinformatics t ools to aide sequencing of difficult templates.

ISBN: 9780763742973 (alk. paper) 076374297X (alk. paper)

LCCN: 2007-042261

Subjects--Topical Terms:
DNA-- analysis.
Nucleotide sequence-- Methodology.
Sequence Analysis, DNA-- methods.
Base Sequence.
Templates, Genetic.

Dewey Class. No.: 572.8/6